In an Attempt to Define the Protein Domains of Protein

question 39

Essay

In an attempt to define the protein domains of protein X, you treat it with a protease and use polyacrylamide gel electrophoresis to analyze the peptides produced.In the past, you have used chymotrypsin to perform this experiment, but the stock of this enzyme has been used up.You find a stock of elastase and decide to use it instead of waiting for a new stock of chymotrypsin to arrive.
A.Give two reasons why elastase is a good substitute for chymotrypsin in this assay.
B.Why might proteolysis of the same substrate by chymotrypsin or elastase yield different results?

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Definitions:

NPV

Net Present Value, a financial metric that calculates the difference between the present value of cash inflows and outflows over a period of time.

Opportunity Cost

The loss of potential gain from other alternatives when one alternative is chosen.

Interest Rate

The cost of borrowing money or the payment received for deposit funds, usually expressed as an annual percentage of the principal.

Wine Barrels

Standardized wooden or metal barrels used for aging, storing, and transporting wine.