Studies Conducted with a Lysozyme Mutant That Contains an Asp→Asn

question 12

Multiple Choice

Studies conducted with a lysozyme mutant that contains an Asp→Asn change at position 52 and a Glu→Gln change at position 35 exhibited almost a complete loss in enzymatic activity.What is the most likely explanation for the decrease in enzyme activity in the mutant?

• A)increased affinity for substrate
• B)absence of negative charges in the active site
• C)change in the active-site scaffold
• D)larger amino acids in the active site decreases the affinity for substrate

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