In an Attempt to Define the Protein Domains of Protein

question 39

Essay

In an attempt to define the protein domains of protein X, you treat it with a protease and use polyacrylamide gel electrophoresis to analyze the peptides produced.In the past, you have used chymotrypsin to perform this experiment, but the stock of this enzyme has been used up.You find a stock of elastase and decide to use it instead of waiting for a new stock of chymotrypsin to arrive.
A.Give two reasons why elastase is a good substitute for chymotrypsin in this assay.
B.Why might proteolysis of the same substrate by chymotrypsin or elastase yield different results?

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Definitions:

Excitatory Conditioned Response

A learned response that increases the probability of a specific behavior in anticipation of a reward or outcome.

Forward Conditioning

A type of classical conditioning where the conditioned stimulus is presented before the unconditioned stimulus, leading to an association between the two.

Rescorla-Wagner Model

A model of conditioning that emphasizes the importance of the predictability of the US by the CS, proposing a limit to the amount of learning that can occur.

Associative Strength

The measure of the relationship between a conditioned stimulus and an unconditioned stimulus, indicating how strongly two stimuli are linked in an individual's mind.