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Researchers Have Isolated a Mutant Strain of E

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Researchers have isolated a mutant strain of E.coli that carries a temperature-sensitive variant of the enzyme DNA ligase.At the permissive temperature, the mutant cells grow just as well as the wild-type cells.At the nonpermissive temperature, all of the cells in the culture tube die within 2 hours.DNA from mutant cells grown at the nonpermissive temperature for 30 minutes is compared with the DNA isolated from cells grown at the permissive temperature.The results are shown in Figure 6-59, where DNA molecules have been separated by size by means of electrophoresis (P, permissive; NP, nonpermissive).Explain the appearance of a distinct band with a size of 200 base pairs (bp) in the sample collected at the nonpermissive temperature. Researchers have isolated a mutant strain of E.coli that carries a temperature-sensitive variant of the enzyme DNA ligase.At the permissive temperature, the mutant cells grow just as well as the wild-type cells.At the nonpermissive temperature, all of the cells in the culture tube die within 2 hours.DNA from mutant cells grown at the nonpermissive temperature for 30 minutes is compared with the DNA isolated from cells grown at the permissive temperature.The results are shown in Figure 6-59, where DNA molecules have been separated by size by means of electrophoresis (P, permissive; NP, nonpermissive).Explain the appearance of a distinct band with a size of 200 base pairs (bp) in the sample collected at the nonpermissive temperature.    Figure 6-59
Figure 6-59

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Definitions:

Liability

A legal responsibility or obligation, often involving financial compensation for damage or harm caused.

Prudently

Acting with or showing care and thought for the future, typically in the management of resources or decision-making processes.

Repair Costs

Expenses incurred to fix or restore damaged or worn-out goods or property to their original condition.

Legal Action

A process by which a court of law is engaged to pursue a legal remedy, often involving litigation.

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