You Prepare Bacterial Cell Extracts by Lysing the Cells and Removing

question 49

Multiple Choice

You prepare bacterial cell extracts by lysing the cells and removing insoluble debris via centrifugation.These extracts provide the proteins required for DNA replication.Your DNA template is a small, double-stranded circular piece of DNA (a plasmid) with a single origin of replication and a single replication termination site.The termination site is on the opposite side of the plasmid from the origin. Figure 6-11
What part of the DNA replication process would be most directly affected if a strain of bacteria lacking helicase were used to make the cell extracts?

• A)initiation of DNA synthesis
• B)Okazaki fragment synthesis
• C)leading-strand elongation
• D)lagging-strand completion

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Definitions:

Neutrality Acts

A series of laws passed by the United States in the 1930s aimed at preventing American involvement in future foreign conflicts by limiting trade and travel with nations at war.

Fascist Aggression

The expansionist policies and acts of aggression carried out by fascist regimes, notably those of Germany and Italy in the lead-up to and during World War II.

Spanish Civil War

A military conflict from 1936 to 1939 between the Republicans, who were loyal to the democratic Spanish Republic, and the Nationalists, a rebel group led by General Francisco Franco.

Francisco Franco

A Spanish general who led the Nationalist forces to victory in the Spanish Civil War and governed Spain as a dictator from 1939 until his death in 1975.