You Have Discovered an "Exo^-" Mutant Form of DNA Polymerase

question 19

Multiple Choice

You have discovered an "Exo^-" mutant form of DNA polymerase in which the 3′-to-5′ exonuclease function has been destroyed but the ability to join nucleotides together is unchanged.Which of the following properties do you expect the mutant polymerase to have?

• A)It will polymerize in both the 5′-to-3′ direction and the 3′-to-5′ direction.
• B)It will polymerize more slowly than the normal Exo⁺ polymerase.
• C)It will fall off the template more frequently than the normal Exo⁺ polymerase.
• D)It will be more likely to generate mismatched base pairs.

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