You prepare bacterial cell extracts by lysing the cells and removing insoluble debris via centrifugation.These extracts provide the proteins required for DNA replication.Your DNA template is a small, double-stranded circular piece of DNA (a plasmid) with a single origin of replication and a single replication termination site.The termination site is on the opposite side of the plasmid from the origin. 
Figure 6-11A
In addition to the extracts and the plasmid DNA, are there any additional materials you should add to this in vitro replication system? Explain your answer.
Definitions:
Aerobic Exercise
Physical activity that increases the heart rate and the body’s use of oxygen, thereby improving overall fitness.
Serotonin
A neurotransmitter that is involved in mood regulation, appetite control, and sleep among other physiological processes.
Stressful Environment
A situation or setting that causes significant anxiety, tension, or stress.
Optimistic
Having a positive or hopeful attitude towards the future, often with a confidence in successful outcomes.
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