You Are Interested in Understanding the Gene Regulation of Lkp1

question 5

Multiple Choice

You are interested in understanding the gene regulation of Lkp1, a protein that is normally produced in liver and kidney cells in mice.Interestingly, you find that the LKP1 gene is not expressed in heart cells.You isolate the DNA upstream of the LKP1 gene, place it upstream of the gene for green fluorescent protein (GFP) , and insert this entire piece of recombinant DNA into mice.You find GFP expressed in liver and kidney cells but not in heart cells, an expression pattern similar to the normal expression of the LKP1 gene.Further experiments demonstrate that there are three regions in the promoter, labeled A, B, and C in Figure 8-16, that contribute to this expression pattern.Assume that a single and unique transcription factor binds each site such that protein X binds site A, protein Y binds site B, and protein Z binds site C.You want to determine which region is responsible for tissue-specific expression, and create mutations in the promoter to determine the function of each of these regions.In Figure 8-16, if the site is missing, it is mutated such that it cannot bind its corresponding transcription factor. Figure 8-16
In what tissue is factor Z normally present and bound to the DNA?

• A)kidney
• B)liver
• C)heart
• D)none of these answers are correct.

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Definitions:

Tidal Volume

The volume of air moved into or out of the lungs during a normal breath at rest.

Inspiratory Reserve

The additional amount of air that can be inhaled after a normal inhalation, demonstrating the reserve capacity of the lungs.

Expiratory Reserve

The additional amount of air that can be expelled from the lungs after a normal exhalation.

Internal Nares

The internal nares, also known as the choanae, are openings at the back of the nasal cavity leading into the pharynx, facilitating airflow from the nose to the throat.