PCR Involves a Heating Step, Followed by a Cooling Step

question 36

Multiple Choice

PCR involves a heating step, followed by a cooling step, and then DNA synthesis.What is the primary reason for why this cooling step is necessary?

• A)Cooling the reaction ensures the integrity of the covalent bonds holding the nucleotides together in the DNA strand.
• B)Cooling the reaction gives the DNA polymerase an opportunity to rest from the previous cycle so that it will be ready for the next round of synthesis.
• C)Transcription takes place during the cooling step.
• D)Cooling the reaction brings the temperature down to a level that is compatible with the short primers forming stable hydrogen bonds with the DNA to be amplified.

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