With Fully Automated Sanger Sequencing, All Four Chain-Terminating DdNTPs Are

question 39

Multiple Choice

With fully automated Sanger sequencing, all four chain-terminating ddNTPs are added into a single reaction and loaded onto a capillary gel.The sequence can be determined even though all ddNTPs are mixed together because

• A)the Sanger sequencing reactions do not contain dNTPs and use the ddNTPS to synthesize the DNA.
• B)the Sanger sequencing reactions utilize ddNTPs, each labeled with a different fluorescent tag, which allows all four ddNTPs to be incorporated into a single molecule of DNA.
• C)the Sanger sequencing reactions generate a set of products, each of which carries a single fluorescent tag whose color reveals the identity of the base that is at the end of the product.
• D)the fully automated Sanger sequencing reactions do not require DNA polymerase because the bases are read as the DNA is pulled through a tiny pore at the end of the capillary gel.

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