You Have the Amino Acid Sequence of a Protein and Wish

question 30

Essay

You have the amino acid sequence of a protein and wish to design PCR primers that will allow you to amplify this gene by PCR from genomic DNA.Using this protein sequence, you deduce a particular DNA sequence that can encode this protein.Why is it unwise to use only this DNA sequence you have deduced to design PCR primers for isolating the gene encoding your protein of interest from genomic DNA?

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Definitions:

Capitated Rates

A payment arrangement for healthcare service providers where they are paid a set amount for each enrolled person assigned to them, regardless of whether that person seeks care.

Unnecessary Costs

Expenses that could be avoided without a detrimental effect on the quality of goods or services offered.

Selective Breeding

The process of breeding plants or animals for specific genetic traits, with the aim of producing offspring with desired characteristics.

Genetic Qualities

Inherent attributes or characteristics passed from parents to offspring through genes.