PCR Involves a Heating Step, Followed by a Cooling Step

question 36

Multiple Choice

PCR involves a heating step, followed by a cooling step, and then DNA synthesis.What is the primary reason for why this cooling step is necessary?

• A)Cooling the reaction ensures the integrity of the covalent bonds holding the nucleotides together in the DNA strand.
• B)Cooling the reaction gives the DNA polymerase an opportunity to rest from the previous cycle so that it will be ready for the next round of synthesis.
• C)Transcription takes place during the cooling step.
• D)Cooling the reaction brings the temperature down to a level that is compatible with the short primers forming stable hydrogen bonds with the DNA to be amplified.

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Definitions:

Transference

A tendency to map on, or transfer, feelings for a person who is known onto someone new who resembles that person in some way.

Physical Resemblance

The similarity in appearance between two or more entities, often referring to genetic or family traits.

Familiar Person

An individual who is recognized or well-known, often through previous encounters or established relationships.

Prosopagnosia

A neurological disorder characterized by the inability to recognize faces, also known as face blindness.