Some Plasmids from CDNA Libraries Can Have Defects Because of the Way

question 48

Essay

Some plasmids from cDNA libraries can have defects because of the way in which a cDNA library is constructed.For each dilemma (A to D) listed below, indicate which of the outcomes (1 to 4) you might encounter, and explain why.Outcomes may be used more than once. $\begin{array}{llcc}\underline{ \text { Dilemma }\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad} & \underline{ \text { Outcomes\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad} }\\ \text { (A) The mRNA corresponding to the clone you are } &\text { (1) The 5' part of the gene will be missing. } \\ \text { looking for was degraded at its 5' end by a } &\\ \text { nuclease.} &\\\\ \text {(B) The mRNA corresponding to the clone you are } & \text {(2) The \( 3^{\prime} \) part of the gene will be missing. }\\ \text { looking for was degraded at its \( 3^{\prime} \) end by a } &\\ \text { nuclease. } & \\ \\ \text { (C) The S'end of the reverse transcriptase product of } & \text {(3) An internal fragment of the gene will be missing }\\ \text { the gene you are trying to clone hybridizes to } &\\ \text { sequences in the middle of the gene. } &\\\\ \text {(D) The gene you are trying to clone has a long } & \text { (4) The gene will be missing from the library.}\\ \text {stretch of A's in the middle of the coding } &\\ \text { sequence. } &\\\end{array}$

Table 10-54

Differentiate between different bases of power and influence tactics, including coercive power and rewards.

Appreciate the significance of ethical considerations in organizational politics and influence processes.

Understand the consequences of political behavior for individuals and organizations, including promotion opportunities and job security.

Identify strategies for workers to effectively manage and navigate organizational politics.

---

Definitions:

Substitutes

Alternative goods or services that can satisfy the same needs as another, often leading to a choice between the two based on price, convenience, or preference.

Perfectly Competitive

A market structure characterized by a large number of small firms, a homogeneous product, free entry and exit, and perfect knowledge, where no single firm can influence the market price.

Profit-Maximizing

The method through which a company identifies the pricing and production quantity that maximizes its profits.

Economic Standpoint

A perspective or analysis based on economic principles, often considering factors like scarcity, supply and demand, and cost-benefit analysis.