Indicate Whether a CDNA Library or a Genomic DNA Library

question 10

Essay

Indicate whether a cDNA library or a genomic DNA library will be more appropriate for use in the following applications.
A.You want to study the promoter of gene A.
B.Gene A encodes a tRNA and you wish to isolate a piece of DNA containing the full-length sequence of the tRNA.
C.You discover that gene A is alternatively spliced and you want to see which predicted alternative splice products the cell actually produces.
D.You want to find both gene A and the genes located near gene A on the chromosome.
E.You want to express gene A in bacteria to produce lots of protein A.

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Definitions:

Isolating Mechanism

Biological features or behaviors that prevent species from interbreeding, thereby maintaining species boundaries.

Delphinium Nudicaule

A species of perennial flower in the buttercup family known for its vivid red-orange flowers, commonly referred to as the red larkspur.

Punctuated Equilibrium

Model of evolutionary change in a species which suggests that there are long periods of little or no change, followed by brief periods of rapid speciation.