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You have the amino acid sequence of a protein and wish to design PCR primers that will allow you to amplify this gene by PCR from genomic DNA.Using this protein sequence, you deduce a particular DNA sequence that can encode this protein.Why is it unwise to use only this DNA sequence you have deduced to design PCR primers for isolating the gene encoding your protein of interest from genomic DNA?
Quality of Design
Refers to the planning and specification of a product's characteristics, aimed to meet customer expectations and requirements from the outset.
Stakeholders
Individuals or groups with an interest or stake in the outcome of a project, including customers, employees, investors, suppliers, and communities.
Benchmarking
The practice of comparing business processes and performance metrics to industry bests and best practices from other companies.
Patient Handoff Quality
Refers to the effectiveness, safety, and efficiency of transferring patient care information and responsibility from one healthcare provider to another.
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