PCR Generates a DNA Fragment of a Distinct Size Even

question 14

Multiple Choice

PCR generates a DNA fragment of a distinct size even when the whole viral chromosome is used as a template. What determines the boundaries of the amplified fragment?

• A) The concentration of one particular deoxynucleotide in the reaction.
• B) The length of time of the elongation step in each cycle.
• C) The position of a termination sequence, which causes the Taq polymerase to fall off the template.
• D) The sites on the template DNA to which the specific primers used in the reaction anneal.
• E) The temperature at which the elongation step in each cycle is carried out.

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Definitions:

Limited Life

A term indicating that an entity or asset has a finite period of usefulness or existence.

General Partner

A partner in a partnership responsible for management decisions, who has unlimited liability for the debts and obligations.

Limited Partnership

A business structure where one or more general partners manage the business and are personally liable, while limited partners invest but do not partake in management or bear as much liability.

Creditor Claims

Rights or legal entitlements of creditors to receive payment or restitution from a debtor.