What Would the Outcome Be If You Forgot to Do

question 39

Multiple Choice

What would the outcome be if you forgot to do the washing step when carrying out FISH on a sample containing bacteria?

• A) Nothing. The washing step is recommended but is not necessary and can be omitted.
• B) Unbound probe would remain on the slide and would fluoresce, making it impossible to see individual cells.
• C) The unbound probe would anneal strongly to the glass slide in random patterns that would look like bacteria. 
• D) The unbound probe would cause lysis of the target bacterial cells, destroying their nuclei and mitochondria.
• E) Nothing. Unbound probe will not fluoresce, so even if it is present, it will not affect the results. 

---

Definitions:

Cash Cycle

The duration between the initial cash outlay for the purchase of inventory and the collection of cash from customers from sales, essentially measuring the liquidity efficiency of a company.

Purchasing Inventory

The process of acquiring goods and materials that a business sells or uses in its production process, an essential component of supply chain management.

Fixed Asset Sale

The process of selling long-term assets, such as property, plant, and equipment, for cash or other compensation.

Cash Increase

A rise in the amount of cash and cash equivalents held by a business.