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Qualitative methods must rely on
Cofactors
Non-protein chemical compounds or metallic ions that are required for an enzyme's activity as a catalyst.
Endergonic Reactions
Chemical reactions that require an input of energy to proceed and are characterized by a positive change in free energy.
Transition State
A high-energy state during a chemical reaction where reactants are converted into products, representing the point of maximum energy during the reaction.
Substrates
A substance or layer that underlies something, or on which some process occurs, in various scientific fields, it often refers to the base on which an organism grows or the substance enzymes act upon.
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