The Rate of an Enzyme-Catalyzed Reaction Initially Increases with an Increase

question 11

Multiple Choice

The rate of an enzyme-catalyzed reaction initially increases with an increase in the substrate concentration,but eventually reaches a maximum value,even though the concentration of substrate continues to increase.Which of the following best explains why?

• A)As substrate concentration increases,the substrates preferentially bind with each other instead of the active site of the enzyme,and no additional catalysis occurs.
• B)As substrate concentration increases,the active sites of all the enzyme molecules become occupied with substrate molecules,and no additional binding can occur.
• C)An increase in substrate concentration eventually increases the temperature of the reaction mixture,and the enzyme becomes denatured.
• D)An increase in substrate concentration allows for greater competition between substrate and inhibitor binding,and enzyme activity ceases.
• E)When substrate concentrations become too high,local variations of pH occur that cause the enzyme to become denatured.

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