Assume That a Given Plasmid Vector to Be Used in a Cloning

question 39

Short Answer

Assume that a given plasmid vector to be used in a cloning experiment contains 4000 base pairs of DNA.Assume also that the restriction endonuclease Cuj cuts this plasmid at the following sites (starting from an arbitrary zero point): 1000,1500,and 3000.Given complete digestion of the plasmid with the endonuclease so that only linear fragments are produced,what sizes of DNA are expected?

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Definitions:

Antiretroviral Therapy

A treatment for HIV/AIDS where a combination of medicines is used to control the virus, reduce its progression, and prevent transmission.

CD4 Counts

A measurement of the number of CD4+ T lymphocytes in the blood, important in assessing the immune function of individuals, especially in the context of HIV infection.

Start

The act of beginning something or initiating an action or process.

HIV

The Human Immunodeficiency Virus is a pathogen that undermines the immune system, potentially culminating in Acquired Immunodeficiency Syndrome (AIDS) in the absence of medical treatment.