Assume That a Given Plasmid Vector to Be Used in a Cloning

question 39

Short Answer

Assume that a given plasmid vector to be used in a cloning experiment contains 4000 base pairs of DNA.Assume also that the restriction endonuclease Cuj cuts this plasmid at the following sites (starting from an arbitrary zero point): 1000,1500,and 3000.Given complete digestion of the plasmid with the endonuclease so that only linear fragments are produced,what sizes of DNA are expected?

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Definitions:

Lever Pressing

A behavior often referred to in psychological experiments, where an animal or person is trained to press a lever to receive a reward or avoid a punishment.

Food Pellets

Small, compressed units of food often used as a convenient way to feed animals or as part of controlled experiments in behavioral studies.

Avoidance Learning

A learning process where a subject learns to prevent or avoid an unpleasant stimulus by performing a particular behavior.

Negative Reinforcement

A behavioral psychology term referring to the removal of an unpleasant stimulus to increase the likelihood of a behavior being repeated.