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The Table Below Lists Several Genotypes Associated with the Lac β\beta

question 18

Essay

The table below lists several genotypes associated with the lac operon in E. coli. For each, indicate with a "+" or a "-" whether functional β-galactosidase would be expected to be produced at induced levels. Genotype                 β\beta -galactosidase production
                                No Lactose         With Lactose
(a) I+O+Z+/FIO+Z+\quad I + O + Z + / F ^ { \prime } I - O + Z +
(b) IOcZ+/FI=O+Z\quad I - O c Z + / F ^ { \prime } I = O + Z -
(c) Is Oc Z+/FI+O+Z+\mathrm { Z } + / \mathrm { F } ^ { \prime } \mathrm { I } + \mathrm { O } + \mathrm { Z } +
(d) IO+Z+/FIO+Z+I - O + Z + / F ^ { \prime } I - O + Z +
I+=I ^ { + } = wild-type repressor
I=I ^ { - } = mutant repressor (unable to bind to the operator)
IsI s = mutant repressor (insensitive to lactose)
O+=O ^ { + } = wild-type operator
OC=O C = constitutive operator (insensitive to repressor)


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