The Following Table Presents the Effect of Different Media on the Growth

question 5

Essay

The following table presents the effect of different media on the growth response of tryptophan mutations in Salmonella typhimurium (+ = growth, - = no growth).

		Medium Supplemented with
Strain	No Supplement	IGP	AA	IN	TRY
trp-8	-	+	+	+	+
trp-2	-	+	-	+	+
trp-3	-	-	-	+	+
trp-1	-	-	-	-	+

(a) Construct the biochemical pathway for the compounds IGP, AA, IN, and TRY based on these data.
(b) Place strains of bacteria (mutations) in the appropriate steps in the pathway.
(c) In bacteria it is often possible to make partial diploid strains. Assume that a diploid strain was made containing the complete genomes of the trp-2 and trp-1 strains. Would this diploid strain be able to grow on the unsupplemented medium? Explain your answer.

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Definitions:

Urinary

Relating to urine or the organs of the urinary system, which includes the kidneys, ureters, bladder, and urethra.

Acidity

A measure of the concentration of hydrogen ions in a solution, reflecting its ability to donate protons and determining its pH level.

Alkalinity

the measure of the capacity of water or a solution to neutralize acids, often indicated by its pH level.

Concentration

In chemistry, the measure of the amount of a given substance contained in a unit volume of a liquid or solid material.