How Does Phenyl Isothiocyanate, Ph−N=C=S, React with a Peptide in the Edman

question 8

Multiple Choice

How does phenyl isothiocyanate, Ph−N=C=S, react with a peptide in the Edman degradation?

• A) the sp carbon acts as an electrophile in a reaction with an amino group of the peptide
• B) the sulfur acts as a nucleophile and adds to the carbon of the peptide bond
• C) the nitrogen acts as a nucleophile and adds to the carbon of the peptide bond
• D) the sp carbon acts as an electrophile in a reaction with a carboxylate of the peptide

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