In the Sanger,or Chain Termination,method of DNA Sequencing,incorporation of the Nucleotides

question 34

Multiple Choice

In the Sanger,or chain termination,method of DNA sequencing,incorporation of the nucleotides stops because

• A)the dideoxynucleotide analogs have no 3ʹ-OH group to continue DNA synthesis.
• B)the dideoxynucleotide analogs have no 5ʹ-OH group to continue DNA synthesis.
• C)the dideoxynucleotide analogs have no 2ʹ-OH group to continue DNA synthesis.
• D)the mixture is heated to 92°C to stop DNA synthesis at specific time intervals.
• E)the dideoxynucleotide analogs are in greater concentration than the actual nucleotides.

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