You Have Determined the Base Sequence for a Gene from Felis

question 48

Multiple Choice

You have determined the base sequence for a gene from Felis catus (domestic cat) that you believe may be involved in feline leukemia.The nucleotide and probable amino acid sequences are similar to retroviral proteins that become embedded in the host cell membrane.You separate the membrane fraction of feline leukemia cell lysate from the cytoplasmic fraction.What technique could you use to determine if your protein of interest is present in the membrane fraction?

• A)affinity chromatography
• B)liquid chromatography followed by mass spectrometry
• C)Southern blot analysis
• D)two-dimensional gel electrophoresis
• E)liquid chromatography followed by mass spectrometry or two-dimensional gel electrophoresis

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