You Are Given the Task of Restriction-Mapping a Portion of a 10

question 11

Essay

You are given the task of restriction-mapping a portion of a 10 kb linear genome fragment from a virus.You digest the fragment with BamHI and,upon gel electrophoresis,observe bands corresponding to 4 kb,5 kb and 1 kb.When you digest the fragment with the enzyme HindII,you observe bands corresponding to 4 kb and 3 kb.When you digest the 10 kb fragment with both enzymes,you observe bands corresponding to 3 kb,2 kb,and 1 kb,although the 2 kb band is much brighter than the other two bands.Based on this information,draw the restriction map of the 10 kb fragment.