You Isolate a New Bacterium from Soil with a Revolutionary

question 55

Multiple Choice

You isolate a new bacterium from soil with a revolutionary new culture technique.You are interested in sequencing the genome of this organism using the whole-genome,or shotgun,sequencing approach.Of the following choices,what would be the best way to prepare the genomic DNA for sequencing?

• A)digestion with HpaI (CCGG)
• B)digestion with BamHI (GGATCC)
• C)digestion with EcoRI (GAATTC)
• D)digestion with NotI (GCGGCCGC)
• E)digestion with EcoRI,BamHI,and HpaI

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Definitions:

Dangerous

A description of something that could potentially cause harm or injury.

Psychiatric Distress

A state of emotional suffering often related to mental health issues, necessitating professional psychiatric intervention.

Minority Status

A social position held by members of a societal group that is systematically disadvantaged or marginalized.

PTSD

Post-Traumatic Stress Disorder, a mental health condition triggered by experiencing or witnessing a terrifying event, characterized by severe anxiety, flashbacks, and nightmares.