You Have Isolated a Piece of DNA That You Believe

question 48

Multiple Choice

You have isolated a piece of DNA that you believe contains an interesting gene.Using a mix of RNA polymerase and ribonucleotides,you perform in vitro transcription.However,even though all of your controls work,no mRNA is created from your DNA fragment.Working backward,you note that your DNA preparation removed approximately 250 base pairs from the 5' end of the gene.The most likely explanation is that during DNA preparation

• A)the termination signal was removed.
• B)the RNA polymerase became inactivated.
• C)the 5' methyl guanosine cap was removed.
• D)the promoter was removed.
• E)None of these explains the negative results.

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Definitions:

Morally Superior

The belief or attitude that one's moral standards, actions, or beliefs are more virtuous or ethical than those of others.

Common Reaction

A typical or widespread response or behavior that people exhibit in a specific situation.

Concern

Concern refers to a feeling of worry or care about a particular issue or situation, often prompting individuals to take action or seek solutions.

Creative Thinking

The ability to think in novel and unconventional ways in order to come up with unique solutions to problems or to generate original ideas.