How Are Temperature Sensitive Mutants Used to Determine When Genes

question 11

Multiple Choice

How are temperature sensitive mutants used to determine when genes act during development?

• A)Embryos can be shifted to the restrictive temperature to inactivate the mutant gene and the effect of inactivation on subsequent stages can be observed.
• B)Embryos can be shifted to the permissive temperature to inactivate the mutant gene and the effect of inactivation on subsequent stages observed.
• C)Embryos can be incubated at the restrictive temperature for several hours.If the embryo develops normally, then the gene product is required during that period of development.
• D)Embryos can be raised at the permissive temperature and if the embryo does not die, then the gene product is not required for development.

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