Using the CRISPR/Cas9 System, a Genetically-Engineered SgRNA Complementary to a Target

question 17

Multiple Choice

Using the CRISPR/Cas9 system, a genetically-engineered sgRNA complementary to a target site in the genome binds to Cas9 endonuclease.A mutation can occur at this site when

• A)Cas9 cleaves the DNA and nonhomologous end-joining results in a small deletion.
• B)Cas9 cleaves the DNA and homologous recombination with the homologous chromosome is used to repair the break.
• C)Cas9 cleaves the DNA to remove the complementary sequence.
• D)Cas9 cleaves the DNA and nonhomologous end-joining correctly repairs the break.

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