To Create a Knockout Mouse, After Introducing to ES Cells

question 2

Multiple Choice

To create a knockout mouse, after introducing to ES cells a DNA construct in which a specific gene is mutagenized by the insertion of a drug resistance marker, the cells

• A)that have incorporated the transgene by homologous recombination grow in the presence of the drug and these are injected into a host blastocyst.
• B)that lack the transgene grow in the presence of the drug and these are injected into a host blastocyst.
• C)that have deleted the transgene grow in the presence of the drug and the nuclei are injected into a host blastocyst.
• D)that lack the transgene grow in the presence of the drug and the nuclei are used for nuclear transfer.

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Definitions:

Codon

A sequence of three nucleotides which together form a unit of genetic code in a DNA or RNA molecule, specifying a particular amino acid.

Amino Acids

Organic compounds that combine to form proteins, acting as the building blocks of life.

Metabolic Defects

Metabolic defects refer to abnormalities in the body’s processes that convert food into energy, leading to various health issues.

Enzyme

Enzyme is a protein that acts as a biological catalyst, speeding up chemical reactions in living organisms without being consumed in the process.