Which Is the Best Method to Demonstrate the Function of a Putative

question 11

Multiple Choice

Which is the best method to demonstrate the function of a putative enhancer?

• A)Clone a region of DNA with the putative enhancer into a reporter construct, introduce the construct into eukaryotic cells, and measure expression of the reporter protein.
• B)Compare DNA sequences from two or more species and identify conserved regions located outside of coding regions.
• C)Introduce two separate pieces of DNA into a eukaryotic cell; one is a fragment of DNA with the putative enhancer and the other is a plasmid that contains a reporter gene.
• D)Clone the putative enhancer into a reporter construct and measure the level of reporter gene expression in a prokaryotic cell.
• E)Crosslink genomic DNA and associated proteins, fragment the DNA, use an antibody to isolate DNA sequences bound to a particular transcription factor, and sequence the DNA.

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