The Episome in an Hfr Strain Is Inserted near the Trp

question 30

Multiple Choice

The episome in an Hfr strain is inserted near the trp (tryptophan operon) locus.This Hfr strain is grown with an F^- Trp^- strain.From this mating, we isolate an F⁺ Trp⁺ strain that can readily impart the Trp⁺ phenotype to F^- Trp^- strains, but no other traits are ever transferred.(When the original Hfr strain is mated with F⁻ strains, traits other than Trp⁺ can be transferred, although at lower frequency than Trp⁺. ) What most likely has happened?

• A)An F′ trp⁺ plasmid has been generated.
• B)Mutation occurred.
• C)Transduction occurred.
• D)Transformation occurred.

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