To Produce a Mutation in a Specific E

question 23

Multiple Choice

To produce a mutation in a specific E.coli gene, an engineered fragment of DNA in which sequences from each end of that gene flank an antibiotic resistance gene

• A)is transformed into cells and replicates independently of the genomic DNA because it has a centromere.
• B)is transformed into cells and maintained as a plasmid.
• C)is introduced into cells and transposes into the bacterial gene.
• D)is introduced into cells and undergoes homologous recombination with the bacterial gene.

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