The Normal Functions of Spo11 and Cas9 Enzymes Are Different

question 60

Multiple Choice

The normal functions of Spo11 and Cas9 enzymes are different because

• A)Spo11 produces double-strand breaks during meiosis, whereas Cas9 functions during mitosis.
• B)Spo11 produces site-specific double-strand breaks during meiosis, whereas Cas9 breaks DNA at random genomic locations.
• C)Spo11 produces double-strand breaks in many genomic locations during meiosis, whereas Cas9 is targeted to specific sequences by a CRISPR RNA.
• D)Spo11 produces double-strand breaks at sequences targeted by RNAs expressed during meiosis, whereas Cas9 has the ability to recognize DNA sequences without an RNA.
• E)Spo11 is unique to yeast, whereas Cas9 occurs in most higher eukaryotes.

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