Polymerase Chain Reaction (PCR) Is Useful for Amplifying a Small

question 32

Multiple Choice

Polymerase chain reaction (PCR) is useful for amplifying a small piece of DNA but is not a good technique for cloning genes because

• A) DNA ligase cannot be used in PCRs.
• B) PCRs do not generate palindromic sequences.
• C) copies of DNA produced by PCRs lack the regulatory genes and proteins required to activate genes.
• D) PCRs only generate primary transcripts that require excision of introns.
• E) ribosomes are required to produce active genes.

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Definitions:

Axons

are long, slender projections of nerve cells (neurons) that transmit electrical impulses away from the neuron’s cell body.

Voltage Dependent

Referring to a process or mechanism that is influenced or activated by changes in electrical potential across a membrane.

Action Potential

A sudden change in electrical charge across a cell membrane, resulting in a rapid signal that can travel along neurons.

Cell Membrane

A biological membrane that separates the interior of all cells from the outside environment, controlling the passage of substances.