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Scenario in Order to Test the Susceptibility of Bacteria (If the the Bacteria

question 34

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Scenario
In order to test the susceptibility of bacteria (if the bacteria are susceptible to being killed) to an antibiotic, a common laboratory test called the Bauer-Kirby Disk Diffusion test is used. First, a petri dish (a shallow, circular, transparent dish with a flat lid) is partially filled with nutrient-enriched agar (a gelatinous material) . The nutrients encourage growth of specific bacterial species. The agar is poured as liquid but then typically solidifies to a soft consistency. Once the agar is set, a "lawn" of bacteria is spread such that a thin layer covers the top of the agar. Antibiotics are then applied to the lawn in a small dose, often through small, saturated paper disks. The dish is typically incubated for 24-48 hours and then observed for susceptibility. If the antibiotic is effective, it will kill the bacteria and leave a clear area called the "zone of inhibition" due to inhibited bacterial growth. The area starts at the source of the antibiotic and radiates outward. Scientists measure the diameter of the zone and then compare it to an established "cutoff value" for a zone specific to antibiotic/organism combinations. A large zone of inhibition in comparison to the standard indicates susceptibility while a small or no zone indicates resistance. Consider the following image, and answer the questions that follow: Scenario In order to test the susceptibility of bacteria (if the bacteria are susceptible to being killed) to an antibiotic, a common laboratory test called the Bauer-Kirby Disk Diffusion test is used. First, a petri dish (a shallow, circular, transparent dish with a flat lid) is partially filled with nutrient-enriched agar (a gelatinous material) . The nutrients encourage growth of specific bacterial species. The agar is poured as liquid but then typically solidifies to a soft consistency. Once the agar is set, a  lawn  of bacteria is spread such that a thin layer covers the top of the agar. Antibiotics are then applied to the lawn in a small dose, often through small, saturated paper disks. The dish is typically incubated for 24-48 hours and then observed for susceptibility. If the antibiotic is effective, it will kill the bacteria and leave a clear area called the  zone of inhibition  due to inhibited bacterial growth. The area starts at the source of the antibiotic and radiates outward. Scientists measure the diameter of the zone and then compare it to an established  cutoff value  for a zone specific to antibiotic/organism combinations. A large zone of inhibition in comparison to the standard indicates susceptibility while a small or no zone indicates resistance. Consider the following image, and answer the questions that follow:   -The Federal Drug Administration (FDA) must decide which antibiotic to approve to fight a bacterial infection that results in high fevers and localized rashes. Which antibiotic do you predict that the FDA scientists will likely approve? A) A because it has the smallest zone of inhibition. B) C because it has the largest zone of inhibition C) E because it has a zone of inhibition similar to A D) F because it does not have a zone of inhibition
-The Federal Drug Administration (FDA) must decide which antibiotic to approve to fight a bacterial infection that results in high fevers and localized rashes. Which antibiotic do you predict that the FDA scientists will likely approve?


Definitions:

Adjusted Trial Balance

A list of all accounts and their balances after adjusting entries are made, used to prepare financial statements.

Financial Statement Column(s)

Sections or columns in financial reports like balance sheets, income statements, and cash flow statements that present financial data of a business in an organized manner.

Accumulated Depreciation

The total amount of depreciation expense that has been recorded for a fixed asset, reducing its book value on the balance sheet over time.

Income Statement Columns

Sections within an income statement that separate financial data into categories, such as revenues, expenses, and net income, to facilitate analysis and understanding of a company's financial performance.

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