Scenario You Conducted a Series of Experiments to Test Whether the the Expression

question 6

Multiple Choice

Scenario
You conducted a series of experiments to test whether the expression of hexokinase, an important enzyme involved in glycolysis, varies under different conditions. In the first experiment, you measured the production of hexokinase mRNA in cells at three different temperatures: 37°C, 25°C, and 10°C. Your results showed that the mRNA levels for hexokinase were 5× and 20× lower at 25°C and 10°C than at 37°C, respectively. Based on these results, you are going to conduct further experiments to attempt to answer your research question.
-What would be the best experiment to do next to determine if cellular hexokinase protein concentration is temperature dependent?

• A)Measure the amount of hexokinase mRNA in cells at different temperatures.
• B)Measure the rate of transcription of the hexokinase gene in cells at different temperatures.
• C)Measure the amount of mRNA that is broken down in cells at different temperatures.
• D)Measure the rate of translation of the hexokinase mRNA in cells at different temperatures.

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