Scenario You Conducted a Series of Experiments to Test Whether the the Expression

question 23

Multiple Choice

Scenario
You conducted a series of experiments to test whether the expression of hexokinase, an important enzyme involved in glycolysis, varies under different conditions. In the first experiment, you measured the production of hexokinase mRNA in cells at three different temperatures: 37°C, 25°C, and 10°C. Your results showed that the mRNA levels for hexokinase were 5× and 20× lower at 25°C and 10°C than at 37°C, respectively. Based on these results, you are going to conduct further experiments to attempt to answer your research question.
-DNA microarrays can be used to determine relative levels of gene expression between samples. DNA microarrays are usually interpreted by color: green means higher mRNA levels, yellow means equal levels, and red means lower levels. If you ran the samples from the temperature experiment on a DNA microarray using the 25°C experiment as a baseline, what results do you predict you would obtain?

• A)The samples from the 37°C experiment would be green and the samples from the 10°C would be red.
• B)The samples from the 37°C experiment would be red and the samples from the 10°C would be green.
• C)The samples from the 37°C experiment would be green and the samples from the 10°C would be yellow.
• D)The samples from the 37°C experiment would be yellow and the samples from the 10°C would be red.

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Definitions:

Compound

A substance formed from two or more elements chemically united in fixed proportions.

Amides

Organic compounds characterized by a carbonyl group attached to a nitrogen atom.

O-Bromobenzoyl Chloride

An organic compound used in the synthesis of other chemical compounds, characterized by a bromine atom attached to the benzoyl chloride group.

Boiling Point

The temperature at which a substance changes from a liquid to a gas at atmospheric pressure.