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You Want to Insert Your Favorite Gene,which Has Been Digested

question 11

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You want to insert your favorite gene,which has been digested with Ncol endonuclease on both ends,into a vector.Ncol recognizes the DNA sequence C*CATGG.The vector you want to use contains many endonuclease recognition sites,but does not include an Ncol site.Which restriction endonuclease would be the most suitable replacement to digest the vector?


Definitions:

Bobo Doll

The Bobo Doll experiment is a social psychology study conducted by Albert Bandura that illustrated how children learn aggressive behaviors through imitation of adult models.

Control Group

In experiments, a group of subjects who do not receive the experimental treatment and are used as a benchmark to measure how the other tested subjects do.

Aggressive Tendencies

Patterns of behavior aimed at causing harm or injury to others, fear of harm in others, or assertion toward dominance in a social context.

Modeling

A method used in psychology to learn through the observation and replication of others' behaviors.

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