Use the Following to Answer Questions
You Are Studying Epigenetic

question 37

Multiple Choice

Use the following to answer questions
You are studying epigenetic regulation of the Bdnf gene in mice and its association with memory. You train mouse B to avoid an adverse stimulus and then isolate genomic DNA from its brain. Mouse A is untrained and used as a control. You take the brain DNA samples and split each of them into two parts. One part is treated with sodium bisulfite and the other part is left untreated. You then perform PCR for the Bdnf locus and digest the PCR product with the restriction enzyme AccII that cuts DNA at the sequence CGCG. The digested DNA is then separated by agarose gel electrophoresis to obtain the following DNA fragments:
$$\begin{array}{l}\begin{array} { c c } \text { Mouse A (Bisulfite DNA) } & \text { Mouse A (Untreated DNA) } \\\hline 21.2 \mathrm {~kb} & 19.3 \mathrm {~kb} \\7.4 \mathrm {~kb} & 7.4 \mathrm {~kb} \\5.8 \mathrm {~kb} & 5.1 \mathrm {~kb} \\5.7 \mathrm {~kb} & 3.9 \mathrm {~kb} \\4.9 \mathrm {~kb} & 3.5 \mathrm {~kb} \\3.5 \mathrm {~kb} & 2.7 \mathrm {~kb} \\& 2.2 \mathrm {~kb} \\& 1.9 \mathrm {~kb} \\& 1.8 \mathrm {~kb} \\& 0.7 \mathrm {~kb}\end{array}\\\\\begin{array} { c c } \text { Mouse B (Bisulfite DNA) } & \text { Mouse B (Untreated DNA) } \\\hline 19.3 \mathrm {~kb} & 19.3 \mathrm {~kb} \\7.4 \mathrm {~kb} & 7.4 \mathrm {~kb} \\5.7 \mathrm {~kb} & 5.1 \mathrm {~kb} \\5.1 \mathrm {~kb} & 3.9 \mathrm {~kb} \\4.9 \mathrm {~kb} & 3.5 \mathrm {~kb} \\3.5 \mathrm {~kb} & 2.7 \mathrm {~kb} \\1.9 \mathrm {~kb} & 2.2 \mathrm {~kb} \\0.7 \mathrm {~kb} & 1.9 \mathrm {~kb} \\& 1.8 \mathrm {~kb} \\& 0.7 \mathrm {~kb}\end{array}\end{array}$$
-How many AccII sites are found at the Bdnf locus?

• A) 4
• B) 6
• C) 9
• D) 10
• E) 16

---

Definitions:

Work Setting

The physical and social environment in which people perform their jobs.

Hygiene Factors

Factors related to the work environment that can lead to job dissatisfaction if absent, such as salary, company policies, working conditions, and job security.

Job Satisfaction

The degree to which an individual feels positive or negative about a job.

Herzberg

Refers to Frederick Herzberg, a psychologist who is best known for introducing the two-factor theory of motivation, distinguishing between hygiene factors and motivators.