Which of the Following Is NOT True of Bisulfite Sequencing

question 31

Multiple Choice

Which of the following is NOT true of bisulfite sequencing?

• A) Methylated cytosines are unaffected by sodium bisulfite.
• B) DNA is treated with sodium bisulfite, which chemically converts unmethylated cytosines to uracil.
• C) Unmethylated cytosines are detected as thymine during sequencing.
• D) All thymines revealed in DNA sequencing of bisulfite treated DNA were cytosines in the original, untreated DNA.
• E) The sequence of genomic DNA treated with sodium bisulfite is compared to the sequence of untreated genomic DNA.

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