You Have Determined That a Bacterial Strain You Are Working

question 9

Essay

You have determined that a bacterial strain you are working with contains a single type of plasmid. You isolate the plasmid DNA and digest separate portions of it with each of two different restriction enzymes, BamHI and HpaI, and also perform a double digest using both enzymes. You then fractionate the enzyme digests on an agarose gel and stain the gel with ethidium bromide to visualize the restriction fragment patterns. Your results are shown below. Size markers (in nucleotide base pairs) are indicated at the left side of the gel. Using these data, construct a possible restriction map for the plasmid.

---

Definitions:

Attachment

A deep and enduring emotional bond that connects one person to another across time and space, often discussed in the context of relationships between infants and caregivers and adult romantic partnerships.

Exchange Relationships

Social interactions based on the principle of reciprocity, where support and resources are given and received with the expectation of equivalent returns.

Pragmatic Relationships

Connections between individuals that are based on practical or utilitarian considerations, rather than emotional or intrinsic values.

Sternberg's Triangular Theory

A psychological model that describes love in terms of three components: intimacy, passion, and commitment.