Below Are the Steps Involved in the RNA-Seq Process

question 66

Multiple Choice

Below are the steps involved in the RNA-seq process. Place these steps in the CORRECT order. 1. Conversion of the RNA to cDNA
2) Fragmentation and preparation of the cDNAs for sequencing
3) Isolation of the RNA molecules of interest from cells
4) Assembly of the sequencing reads into RNA transcripts
5) Sequencing of the cDNA

• A) 3, 1, 2, 5, 4
• B) 1, 2, 4, 5, 3
• C) 3, 4, 1, 2, 5
• D) 1, 5, 4, 3, 2
• E) 1, 5, 2, 3, 4

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Definitions:

Galactose

A simple sugar that is a component of lactose, found in milk and dairy products, and is metabolized by the body into glucose.

Lateral Hypothalamus

An area of the brain thought to control hunger and thirst; damage can reduce the desire to eat and drink.

Sugar Receptors

Cellular structures that detect the presence of sugar molecules, primarily involved in taste perception.

Ventromedial Hypothalamus

A part of the hypothalamus that plays a role in satiety, or feeling full after eating, and regulating part of the body's energy balance.