A Scientist Edits a Mammalian Genome by Inserting a GFP

question 70

Multiple Choice

A scientist edits a mammalian genome by inserting a GFP reporter sequence downstream of a gene of interest. Which of the following methods is she likely to have used to make this change?

• A) site-directed mutagenesis
• B) oligonucleotide-directed mutagenesis
• C) CRISPR-Cas9 genome editing followed by nonhomologous end joining (NHEJ)
• D) CRISPR-Cas9 genome editing followed by homologous recombination (HR) with a donor template
• E) mutagenesis with radiation

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