Use the Following to Answer Questions

question 30

Multiple Choice

Use the following to answer questions
A scientist carries out a ligation reaction designed to insert a foreign piece of DNA into a plasmid that contains the front end of the lacZ gene within the multiple cloning site (MCS) . He carries out three transformations in parallel with lacZ^- bacteria. The three transformations (i, ii, and iii) contain the following:
i. sterile water
ii. a sample of the original plasmid
iii. the ligation reaction
He then plates the transformations on medium containing X-gal and ampicillin.
-The scientist observed colonies on plate (i) . Which of the following might be reasons(s) for this? (Select all that apply.)

• A) The plasmid he used has a kanamycin resistance marker instead of an ampicillin resistance marker.
• B) He transformed cells that were not actually ampicillin sensitive.
• C) Too high a concentration of ampicillin was added to the plates.
• D) Too low a concentration of ampicillin was added to the plates.
• E) The water sample was contaminated with the original plasmid.

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Definitions:

Indeterminism

The thesis that at least some events in the universe are not determined, not caused by antecedent conditions, and not predictable.

Uncaused Actions

Actions that occur without a preceding cause, often discussed in the context of free will and determinism.

Descartes

A French philosopher, mathematician, and scientist, considered the father of modern philosophy, known for his statement "Cogito, ergo sum" ("I think, therefore I am").

Jean-Paul Sartre

A French existentialist philosopher, playwright, and novelist known for his exploration of freedom, existentialism, and the absurd.