A Gene Does Not Contain the Necessary Restriction Enzyme Sites

question 51

Multiple Choice

A gene does not contain the necessary restriction enzyme sites for cloning into a plasmid vector. What is a possible option?

• A) Increase the amount of gene used.
• B) Add linkers to generate new restriction enzyme sites.
• C) Use a cosmid as a cloning vector.
• D) Use dideoxy sequencing to obtain the sequence of the gene.
• E) Cut the DNA with a blunt end cutter.

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