A Scientist Attempts to Use the CRISPR-Cas9 System to Edit

question 42

Multiple Choice

A scientist attempts to use the CRISPR-Cas9 system to edit a single gene within a cell. She finds that, in addition to editing the desired gene, she has edited several other genes as well. What are reasonable options for her to try next in order to target just the gene of interest? (Select all that apply.)

• A) Add less Cas9 enzyme.
• B) Create a longer sgRNA.
• C) Try a different sgRNA that will still pair within the gene of interest.
• D) Use separate crRNA and tracrRNA molecules.
• E) Use a higher-fidelity version of Cas9.

Comprehend the structure and use of regression equations in predictive analysis.

Understand the significance of path coefficients in SEM.

Identify the steps necessary for predicting future behavior based on current scores.

Interpret the impact of controlling a third variable on correlation coefficients.