Use the Following to Answer Questions

question 64

Multiple Choice

Use the following to answer questions
A student carries out PCR using the following steps:
Step 1: 94°C for 1 minute
Step 2: 60°C for 30 seconds
Step 3: 72°C for 30 seconds
-After subjecting his PCR reaction to gel electrophoresis, the student sees no PCR product on the gel. What error(s) might he have made? (Select all that apply.)

• A) He carried out step 1 at too low a temperature.
• B) He carried out step 1 at too high a temperature.
• C) He carried out step 2 at too low a temperature.
• D) He carried out step 2 at too high a temperature.
• E) He carried out step 3 for too short a time.

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Definitions:

Zone Of Proximal Development

The gap between an individual's independent capabilities and their potential when supported by a knowledgeable companion through advice and motivation.

Alphabet Recognition

The ability to visually identify and name all the letters of the alphabet, a foundational skill in literacy.

Ground Tissue System

All tissues in the plant body other than the dermal tissue system and vascular tissue system; consists of parenchyma, collenchyma, and sclerenchyma

Vascular Tissue System

The tissues specialized for translocation of materials throughout the plant body (i.e., the xylem and phloem).