Use the Following to Answer Questions

question 65

Multiple Choice

Use the following to answer questions
A scientist carries out a ligation reaction designed to insert a foreign piece of DNA into a plasmid that contains the front end of the lacZ gene within the multiple cloning site (MCS) . He carries out three transformations in parallel with lacZ^- bacteria. The three transformations (i, ii, and iii) contain the following:
i. sterile water
ii. a sample of the original plasmid
iii. the ligation reaction
He then plates the transformations on medium containing X-gal and ampicillin.
-What results would indicate the scientist should proceed to culture colonies from (iii) that might contain the desired clone?

• A) Many blue colonies on all three plates.
• B) White colonies on plates (i) and (ii) and predominantly blue colonies on plate (iii).
• C) No colonies on plate (i), white colonies on plate (ii), and predominantly blue colonies on plate (iii).
• D) No colonies on plate (i), blue colonies on plate (ii), and predominantly white colonies on plate (iii).
• E) No colonies on plate (i), blue colonies on plate (ii), and predominantly blue colonies on plate (iii).

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Definitions:

Capital Budgeting

The process by which a business evaluates and selects long-term investment projects.

Cash Flows

The total amount of money being transferred into and out of a business, affecting the liquidity and overall financial health of the company.

Capital Budgeting

The process through which a company evaluates and selects long-term investments that are worth more than their cost to pursue.

Long-Term Projects

Long-term projects involve commitments or investments that span several years, focusing on achieving objectives that contribute to a company's growth or development.